Determination of sulfonamides in livers using matrix solid-phase dispersion extraction high-performance liquid...

Area of Science:

• Analytical Chemistry
• Food Safety
• Pharmacology

Background:

• Sulfonamides (SAs) are widely used antimicrobials in food-producing animals.
• Residue analysis of SAs in edible tissues is crucial for food safety and regulatory compliance.
• Efficient extraction methods are needed for accurate determination of SAs in complex matrices like liver.

Purpose of the Study:

• To develop and optimize a matrix solid-phase dispersion (MSPD) method for extracting seven sulfonamides from liver samples.
• To validate the method for the determination of SAs using high-performance liquid chromatography (HPLC) with fluorescence detection.
• To assess the method's applicability for analyzing SAs in porcine, chicken, and cattle liver.

Main Methods:

• Matrix solid-phase dispersion (MSPD) was employed for sample preparation.
• Diatomaceous earth was identified as the optimal dispersion adsorbent with a 3:1 adsorbent-to-sample ratio.
• Acetone was utilized as the elution solvent.
• High-performance liquid chromatography (HPLC) coupled with a fluorescence detector was used for separation and detection after derivatization with fluorescamine.

Main Results:

• The optimized MSPD method achieved a linear range of 5.0-1000.0 ng/g for SAs in liver samples.
• Average recoveries for the seven SAs exceeded 84.6% in spiked porcine, chicken, and cattle liver samples.
• The method demonstrated good efficiency for extracting sulfonamide residues from complex biological matrices.

Conclusions:

• The developed MSPD method is effective and reliable for the extraction and determination of sulfonamides in liver tissues.
• This approach provides a valuable tool for routine analysis and monitoring of sulfonamide residues in food safety applications.
• The high recovery rates confirm the suitability of MSPD for sensitive detection of sulfonamides in animal-derived food products.