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DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: May 26, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Agilent microRNA microarray profiling system.

Petula N D'Andrade1, Stephanie Fulmer-Smentek

  • 1Agilent Technologies, Santa Clara, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 7, 2011
PubMed
Summary
This summary is machine-generated.

MicroRNA (miRNA) profiling is crucial for understanding cellular regulation and diseases like cancer. The Agilent miRNA profiling system offers a sensitive and accurate microarray assay for precise miRNA detection across various sample types.

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Published on: August 3, 2011

Performing Custom MicroRNA Microarray Experiments
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Related Experiment Videos

Last Updated: May 26, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Performing Custom MicroRNA Microarray Experiments
07:04

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genomics

Background:

  • MicroRNAs (miRNAs) are key regulators of cellular processes.
  • miRNA expression signatures are linked to diseases, notably cancer.
  • Accurate miRNA profiling is essential for biological research and diagnostics.

Purpose of the Study:

  • To introduce and evaluate the Agilent Technologies miRNA profiling system.
  • To highlight the system's sensitivity, accuracy, and broad applicability.
  • To detail the novel labeling and probe design strategies employed.

Main Methods:

  • Utilizes a sensitive and accurate miRNA microarray assay.
  • Employs a single-vial, direct labeling method using Cy3-labeled pCp.
  • Features novel probe design for sequence and size discrimination.
  • Leverages Agilent's flexible microarray design platform.

Main Results:

  • Achieves high specificity in detecting mature miRNAs, even closely related ones.
  • Demonstrates a linear dynamic range spanning over four orders of magnitude (0.2 amol to 2 fmol).
  • Reports a low detection limit of less than 0.1 amol.
  • Validated for use with diverse sample types, including FFPE samples.

Conclusions:

  • The Agilent miRNA profiling system provides precise and accurate miRNA measurements.
  • Its sensitivity, specificity, and flexibility make it suitable for various research applications.
  • The assay enables robust miRNA profiling across different sample matrices.