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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: May 26, 2026

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
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Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

miRNA expression profiling using Illumina Universal BeadChips.

Jing Chen1, Craig S April, Jian-Bing Fan

  • 1Illumina, Inc., San Diego, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 7, 2011
PubMed
Summary
This summary is machine-generated.

A new BeadArray method offers sensitive and reproducible microRNA (miRNA) expression profiling. This technique accurately detects small expression differences, enabling high-throughput analysis of numerous tissue samples.

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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
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Last Updated: May 26, 2026

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
10:37

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • MicroRNA (miRNA) expression profiling is crucial for understanding gene regulation.
  • Existing methods may lack sensitivity, specificity, or reproducibility for high-throughput analysis.
  • Distinguishing between closely related miRNA family members presents a technical challenge.

Purpose of the Study:

  • To develop a novel, highly sensitive, specific, and reproducible method for miRNA expression profiling.
  • To enable accurate quantification of miRNA expression using limited total RNA input.
  • To facilitate high-throughput analysis of miRNA expression in large sample cohorts.

Main Methods:

  • Utilized BeadArray technology for miRNA expression profiling.
  • Incorporated an enzyme-assisted specificity step and solid-phase primer extension.
  • Employed universal PCR amplification prior to array hybridization.

Main Results:

  • Achieved highly reproducible miRNA expression profiles with 100-200 ng total RNA.
  • Demonstrated a dynamic range of 3.5-4 log (10^5-10^9 molecules).
  • Detected 1.2- to 1.3-fold differences between samples with high accuracy.
  • Generated expression profiles comparable to RT-PCR (R^2 = 0.85-0.90) and direct sequencing (R = 0.87-0.89).

Conclusions:

  • The developed BeadArray method provides a sensitive, specific, and reproducible approach for miRNA expression profiling.
  • This method is suitable for high-throughput analysis of miRNA expression in extensive collections of tissue samples.
  • The technique offers a valuable tool for advancing research in miRNA-related biological processes and diseases.