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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: May 26, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

MicroRNA profiling using µParaflo microfluidic array technology.

Xiaochuan Zhou1, Qi Zhu, Christoph Eicken

  • 1LC Sciences, LLC, Houston, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 7, 2011
PubMed
Summary
This summary is machine-generated.

MicroRNA (miRNA) profiling is detailed using microfluidic microarray technology. This method offers flexibility for focused or discovery-based miRNA studies, aiding in disease biomarker research.

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Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Published on: December 3, 2010

Related Experiment Videos

Last Updated: May 26, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
10:58

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Published on: December 3, 2010

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genomics

Background:

  • MicroRNAs (miRNAs) are endogenous short RNA molecules regulating gene expression.
  • Dysregulated miRNA expression is linked to various diseases, making them potential biomarkers.
  • miRNAs are found in cytoplasm and serum fluids, influencing cell division, growth, and apoptosis.

Purpose of the Study:

  • To describe methods for miRNA profiling using μParaflo microfluidic oligonucleotide microarray technology.
  • To highlight the technology's flexibility for both focused and discovery-based miRNA research.
  • To provide comprehensive guidance on experimental design, sample preparation, and data analysis for miRNA array experiments.

Main Methods:

  • Utilized μParaflo microfluidic oligonucleotide microarray technology for miRNA profiling.
  • Emphasized user-defined sequence content selection for tailored experimental needs.
  • Detailed protocols covering experimental design, sample preparation, and data analysis.

Main Results:

  • Demonstrated the application of microfluidic microarray technology for comprehensive miRNA profiling.
  • Showcased the adaptability of the platform for diverse research objectives, from specific target analysis to broad discovery.
  • Provided a complete workflow for conducting miRNA array experiments.

Conclusions:

  • Microfluidic microarray technology offers a flexible and powerful tool for miRNA profiling.
  • The described methods facilitate robust experimental design and data analysis for miRNA research.
  • This approach supports the identification and validation of miRNAs as biomarkers for various disease states.