Gene expression in mouse oocytes by RNA-Seq
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Updated: May 26, 2026
08:00
Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
Published on: June 16, 2021
1The Jackson Laboratory, Bar Harbor, ME, USA. Eric.antoniou@jax.org
Methods in Molecular Biology (Clifton, N.J.)
|December 7, 2011
Abstract:
Second-generation sequencers such as the Illumina GAIIx make possible the study of all transcribed loci in a genome across an almost endless dynamic range. Although typical protocols call for starting from at least 1 μg of total RNA, this is not possible when studying small tissues or rare cell types. This chapter explains how to prepare Illumina sequencing libraries from mouse oocytes. The protocol is also suitable for mural and cumulus cells, flow sorted or laser captured cells.
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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