Related Experiment Video
Updated: May 26, 2026
11:25
Isolation and Culture of Mouse Cortical Astrocytes
Published on: January 19, 2013
Isolation and culture of astrocytes from the retina and optic nerve
1Molecular Pharmacology and Biological Chemistry Ward 8-200, Northwestern University, Chicago, IL, USA. t-lukas@northwestern.edu
Methods in Molecular Biology (Clifton, N.J.)
|December 7, 2011
Abstract:
Detailed methods for the preparation of optic nerve head (ONH) astrocytes from human donor eyes and retinal astrocytes from rat eyes are described. Included is the immunopanning method used for ONH astrocyte isolation as well as cell characterization. The isolation of purified retinal astrocytes is outlined as a method applicable to rodent and other mammalian retinas.
