Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Molecular Factors Affecting Cell Division01:27

Molecular Factors Affecting Cell Division

Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinct roles of the JADE and BRPF scaffolding subunits of the acetyltransferase HBO1 complex.

Nature communications·2026
Same author

An intricate functional relationship between NuA4 and Sfp1 regulates ribosome biogenesis in response to nutrient availability.

The Journal of biological chemistry·2026
Same author

Cryo-EM Structure of Human ATAD2B Reveals a Hexameric Organization Contributes to ATPase Activity and Substrate Coordination.

bioRxiv : the preprint server for biology·2026
Same author

Unraveling the complexity of the histone code: implications for gene regulation and disease.

Genome biology·2026
Same author

Breaking barriers: transitioning from X-ray crystallography to cryo-EM for structural studies.

Acta crystallographica. Section D, Structural biology·2026
Same author

From latent dynamics to data geometry: Nonlinear diffusion modelling for protein structures.

Computational and structural biotechnology journal·2026

Related Experiment Video

Updated: May 26, 2026

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation
05:44

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation

Published on: January 26, 2024

Conserved molecular interactions within the HBO1 acetyltransferase complexes regulate cell proliferation.

Nikita Avvakumov1, Marie-Eve Lalonde, Nehmé Saksouk

  • 1Laval University Cancer Research Center, Hôtel-Dieu de Québec, Quebec City, Quebec, Canada.

Molecular and Cellular Biology
|December 7, 2011
PubMed
Summary

MYST acetyltransferase complexes with ING proteins regulate cell proliferation. Their association, particularly ING4/5 PHD domain with HBO1-JADE, is crucial for tumor suppressor activity and p53 pathway regulation.

More Related Videos

Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Related Experiment Videos

Last Updated: May 26, 2026

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation
05:44

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation

Published on: January 26, 2024

Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Cancer Biology

Background:

  • MYST family acetyltransferase complexes, including HBO1/ING, are vital for gene regulation and DNA replication.
  • These complexes acetylate histones H3 and H4, influencing cellular processes.
  • ING tumor suppressor proteins are conserved partners of MYST acetyltransferases.

Purpose of the Study:

  • To dissect the molecular assembly and functional domains of HBO1/ING complexes.
  • To elucidate the role of specific ING protein domains in complex formation and tumor suppression.
  • To identify the downstream pathways regulated by HBO1/ING complexes.

Main Methods:

  • Molecular dissection of protein domains within HBO1/ING complexes.
  • Analysis of PHD finger domain binding specificity to histone H3.
  • Functional genomic analyses to assess pathway regulation.

Main Results:

  • Multiple PHD finger domains in HBO1/ING subunits bind histone H3 tails, with specificity for lysine 4 methylation.
  • The association of ING4/5 PHD domain with HBO1-JADE is critical for growth inhibition and tumor suppressor function.
  • The complex directly regulates the p53 pathway, including transcription of p21/CDKN1A.

Conclusions:

  • ING association with MYST acetyltransferases is essential for controlling cell proliferation.
  • The regulated interaction between ING proteins and acetyltransferases underpins their tumor suppressor activities.
  • Targeting HBO1/ING complexes offers potential therapeutic strategies for cancer treatment.