Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview of Metabolism

Overview of Metabolism

Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...

Bioreactor Controls-III

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

C4 Pathway and CAM

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...

Phase II Reactions: Methylation Reactions

Phase II Reactions: Methylation Reactions

Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...

The Calvin Benson Cycle

The Calvin Benson Cycle

Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...

Fates of Pyruvate

Fates of Pyruvate

Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Efficacy of distal haemostasis during caesarean delivery in women with placenta accreta spectrum disorders.

The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·2021

Same author

Analysis of risk factors and predictors of pregnancy loss and strategies for the management of cervical insufficiency in pregnant women at a high risk of preterm birth.

The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·2019

Same author

PREDICTORS OF FATAL OUTCOME IN PATIENTS WITH HEMORRHAGIC SHOCK IN GASTROINTESTINAL BLEEDING.

Vestnik khirurgii imeni I. I. Grekova·2018

Same author

[Clinical variations of chronic generalized periodontitis, genetic polymorphism and systemic production of inflammatory cytokines].

Stomatologiia·2016

Same author

[First experience of application of assist circulation device using biventricular type "excor"].

Vestnik khirurgii imeni I. I. Grekova·2014

Same author

[Influence of methanol on the content of NAD(P)H, free amino acids and protein in the cells of Chlamydomonas reinhartdii].

Ukrains'kyi biokhimichnyi zhurnal (1999 )·2013

Search research articles

Related Experiment Video

Updated: May 26, 2026

Elucidating the Metabolism of 2,4-Dibromophenol in Plants

Elucidating the Metabolism of 2,4-Dibromophenol in Plants

Published on: February 10, 2023

[Methanol metabolism in plants].

S S Stepanov, O K Zolotar'ova

Ukrains'Kyi Biokhimichnyi Zhurnal (1999 )

|December 8, 2011

Summary

Plants can metabolize methanol, incorporating its carbon into organic molecules. Low methanol concentrations can enhance plant photosynthetic efficiency by stimulating key metabolic pathways.

Area of Science:

Plant physiology and biochemistry
C1 metabolism
Photosynthesis

Background:

Methanol metabolism in plants is a complex process involving specific enzymes.
Plant organisms uniquely integrate methanol transformation with photorespiration and C1 metabolism.
The carbon from methanol can be utilized for synthesizing organic matter via photosynthesis.

Purpose of the Study:

To describe the enzymes involved in methanol oxidation and carbon incorporation in plants.
To elucidate the interaction between methanol metabolism and other key plant metabolic pathways.
To investigate the effect of exogenous methanol on plant photosynthetic efficiency.

Main Methods:

Enzymatic assays for methanol oxidation.
Metabolic pathway analysis of C1 incorporation.

More Related Videos

Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry

Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry

Published on: May 16, 2014

13C6-Glucose Labeling Associated with LC-MS: Identification of Plant Primary Organs in Secondary Metabolite Synthesis

¹³C₆-Glucose Labeling Associated with LC-MS: Identification of Plant Primary Organs in Secondary Metabolite Synthesis

Published on: March 22, 2024

Related Experiment Videos

Last Updated: May 26, 2026

Elucidating the Metabolism of 2,4-Dibromophenol in Plants

Elucidating the Metabolism of 2,4-Dibromophenol in Plants

Published on: February 10, 2023

Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry

Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry

Published on: May 16, 2014

13C6-Glucose Labeling Associated with LC-MS: Identification of Plant Primary Organs in Secondary Metabolite Synthesis

¹³C₆-Glucose Labeling Associated with LC-MS: Identification of Plant Primary Organs in Secondary Metabolite Synthesis

Published on: March 22, 2024

Measurement of photosynthetic parameters under varying methanol concentrations.

Main Results:

Enzymes responsible for sequential methanol oxidation were identified.
Mechanisms for incorporating methanol-derived carbon into phospholipids, carboxylic acids, and carbohydrates were detailed.
Methanol metabolites, formaldehyde and formiate, are key intermediates in anabolic processes.
Exogenous methanol at low concentrations was found to stimulate plant photosynthetic efficiency.

Conclusions:

Plant methanol metabolism is intricately linked with photorespiration and C1 metabolism.
Plants can effectively utilize methanol carbon for organic matter synthesis through photosynthesis.
Low concentrations of exogenous methanol can positively impact plant photosynthetic performance.