A coordination complex system for generic, ultrafast, and sensitive multimode fluorescent staining of biomolecules

Area of Science:

• Biochemistry
• Molecular Biology
• Analytical Chemistry

Background:

• Existing gel electrophoresis staining methods are often specific to the biomolecule being analyzed.
• There is a need for universal, rapid, and sensitive staining techniques for various biomolecules.

Purpose of the Study:

• To develop a versatile, ultrafast, and sensitive fluorescent staining system for simultaneous identification of DNA, RNA, and proteins.
• To enable rapid image contrast generation and potential high-throughput analysis.

Main Methods:

• Development of a generic, positively charged, planar ligand-based coordination complex.
• Utilizing aggregation-induced fluorescence quenching upon interaction with target biomolecules (DNA, RNA, proteins).
• Employing sequential reactions with cation and ligand for staining, and washing/photobleaching for contrast reversal.

Main Results:

• The system achieved efficient identification of DNA, RNA, and proteins within one minute.
• Demonstrated effective staining efficacy through fluorescence quenching and contrast reversal.
• Showcased versatility across different biomolecule types and staining modes.

Conclusions:

• The developed fluorescent system offers a generic, ultrafast, and sensitive approach for biomolecule detection in gel electrophoresis.
• The multimode capabilities and rapid readout potential pave the way for novel staining strategies and high-throughput applications.