Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Two-dimensional Gel Electrophoresis

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...

Electrospray Ionization (ESI) Mass Spectrometry

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...

Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Charge Selectivity of an Ionic Transistor.

Langmuir : the ACS journal of surfaces and colloids·2021

Same author

Anomalous diffusion in an electrolyte saturated paper matrix.

Electrophoresis·2020

Same author

The effect of the finite size of ions and Debye layer overspill on the screened Coulomb interactions between charged flat plates.

Electrophoresis·2019

Same author

A numerical study of the selectivity of an isolated cylindrical or conical nanopore to a charged macro-ion.

Biomicrofluidics·2019

Same author

Solid-state nanopore hydrodynamics and transport.

Biomicrofluidics·2019

Same author

Band Broadening Theories in Capillary Electrophoresis.

Methods in molecular biology (Clifton, N.J.)·2018

Search research articles

Related Experiment Video

Updated: May 26, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Electromigration dispersion in capillary electrophoresis.

Zhen Chen¹, Sandip Ghosal

¹Dept. Mech. Eng., Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA. zhenchen2011@u.northwestern.edu

Bulletin of Mathematical Biology

|December 8, 2011

Summary

This summary is machine-generated.

This study demonstrates that a nonlinear wave equation governs solute concentration in capillary electrophoresis, even with weak electrolytes. An approximate formula for peak variance shows good agreement with experimental data.

More Related Videos

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

Related Experiment Videos

Last Updated: May 26, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

Area of Science:

Biophysical Chemistry
Analytical Chemistry
Mathematical Biology

Background:

Previous work established a nonlinear wave equation for capillary electrophoresis (CE) solute concentration, simplifying to Burger's equation for weak nonlinearity and strong electrolytes.
The governing equation's applicability to weak electrolytes was previously unaddressed.

Purpose of the Study:

To investigate the applicability of the established nonlinear wave equation to capillary electrophoresis (CE) buffers composed of a single weak acid or base.
To derive and validate an approximate formula for dimensionless peak variance in such systems.

Main Methods:

Mathematical modeling of solute concentration dynamics in CE using a nonlinear wave equation.
Derivation of an approximate analytical formula for dimensionless peak variance.
Comparison of the derived formula with existing experimental data for weak electrolyte systems.

Main Results:

The nonlinear wave equation accurately describes solute concentration evolution in CE with weak electrolytes (single weak acid/base).
A novel, simple approximate formula for dimensionless peak variance was derived.
The derived formula demonstrated strong agreement with published experimental results.

Conclusions:

The nonlinear wave equation is a versatile model for CE, applicable to both strong and weak electrolytes.
The developed approximate formula provides a useful tool for analyzing peak variance in weak electrolyte CE experiments.
This research expands the theoretical framework for understanding CE separation dynamics.