Related Experiment Video
Updated: May 26, 2026
11:47
Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods
Published on: December 9, 2022
Effect of vanadium chloroperoxidase on Enterococcus faecalis biofilms
Ilona F Persoon1, Michel A Hoogenkamp, Aleksandra Bury
1Department of Conservative and Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University, Amsterdam, The Netherlands. i.persoon@acta.nl
Journal of Endodontics
|December 14, 2011
Summary
Vanadium chloroperoxidase (VCPO) reaction products effectively reduce Enterococcus faecalis biofilm viability. This antimicrobial effect suggests potential use in endodontic treatments.
Area of Science:
- Biochemistry
- Microbiology
- Enzyme kinetics
Background:
- Enterococcus faecalis is a common cause of endodontic infections.
- Biofilm formation by E. faecalis presents a significant clinical challenge.
- Novel antimicrobial strategies are needed to combat resistant E. faecalis biofilms.
Purpose of the Study:
- To investigate the antimicrobial activity of vanadium chloroperoxidase (VCPO) reaction products.
- To assess the efficacy of VCPO against four distinct strains of Enterococcus faecalis biofilms.
Main Methods:
- Cultured 24-hour biofilms of E. faecalis strains (V583, ER5/1, E2, OS-16).
- Incubated biofilms with VCPO, halide (bromide or chloride), and hydrogen peroxide.
- Quantified bacterial viability using colony-forming unit counts.
Main Results:
- VCPO reaction products demonstrated significant antimicrobial efficacy, reducing E. faecalis viability by 94% (range 87%-100%).
- Bromide as the halide was more effective than chloride against E. faecalis strains E2 and OS-16.
- VCPO treatment showed consistent effectiveness across all four E. faecalis strains, irrespective of biofilm quantity.
Conclusions:
- VCPO treatment exhibits a notable antimicrobial effect on in vitro E. faecalis biofilms.
- VCPO holds promise as an adjunctive therapy in endodontic treatment.
- Potential application of VCPO as an antimicrobial dressing in endodontics.
Related Concept Videos
01:29Biofilms
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
01:28Antimicrobial Effectiveness
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...