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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...

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Related Experiment Video

Updated: May 26, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Single cell genome sequencing.

Suzan Yilmaz1, Anup K Singh

  • 1Department of Bioengineering and Biotechnology, Sandia National Laboratory, Livermore, CA 94551, United States.

Current Opinion in Biotechnology
|December 14, 2011
PubMed
Summary
This summary is machine-generated.

Single cell sequencing unlocks the study of uncultivated microbes, enabling function-to-species linkage and analysis of low-abundance organisms. This powerful technique is poised to become standard for microbial community genome and transcriptome research.

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Area of Science:

  • Microbiology
  • Genomics
  • Bioinformatics

Background:

  • Uncultivated microorganisms comprise the vast majority of environmental microbes.
  • Metagenomic techniques often fail to identify low-abundance species or link functions to specific microbes.
  • Analyzing single microbial cells offers a solution to these limitations.

Purpose of the Study:

  • To highlight the utility of whole genome amplification and single cell sequencing for microbial research.
  • To demonstrate the advantages of single cell sequencing over traditional metagenomics.
  • To emphasize the potential of single cell sequencing in microbial ecology and genomics.

Main Methods:

  • Whole genome amplification (WGA) of DNA from individual microbial cells.
  • Next-generation sequencing (NGS) of amplified single cell genomes.
  • Bioinformatic analysis for genome assembly, binning, and functional annotation.

Main Results:

  • Single cell sequencing successfully identifies previously uncultivated microorganisms.
  • This method links specific functions to microbial species, a capability lacking in metagenomics.
  • Low-abundance microbial species, often missed in community analyses, can be effectively studied.
  • Single cell sequencing complements metagenomics for improved genome assembly and binning.

Conclusions:

  • Single cell sequencing is a powerful tool for exploring microbial diversity and function.
  • It overcomes key limitations of metagenomic approaches in microbial community studies.
  • Advancements in sequencing technology will likely establish single cell sequencing as a standard method for microbial genomics and transcriptomics.