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Activity measurement of protein kinase and protein phosphatase by microchip phosphate-affinity electrophoresis

Aishan Han1, Kazuo Hosokawa, Mizuo Maeda

  • 1Bioengineering Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.

Analytical Biochemistry
|December 14, 2011
PubMed

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Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
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Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...