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Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies
Published on: September 6, 2017
Chimerism detection by short tandem repeat analysis when donor and recipient genotypes are not known
A Odriozola1, J A Riancho, V Mijares
1Unit of Legal Medicine, University of Cantabria, Santander, Spain. adrianodriozola@gmail.com
Clinica Chimica Acta; International Journal of Clinical Chemistry
|December 14, 2011
Summary
A new panel of 8 short tandem repeat (STR) markers efficiently detects chimerism after bone marrow transplantation. This optimized STR analysis improves donor-recipient identification, even without pre-transplant genotypes.
Area of Science:
- Molecular Biology
- Genetics
- Transplantation Medicine
Background:
- Current short tandem repeat (STR) analysis for bone marrow transplant chimerism uses forensic kits with excessive non-informative STRs.
- Lack of pre-transplant recipient or donor genotypes complicates post-transplant chimerism analysis.
- Existing methods are suboptimal for routine clinical chimerism detection.
Purpose of the Study:
- To develop a more informative STR marker panel for chimerism analysis.
- To enable effective direct detection of chimerism, irrespective of pre-transplant genotype availability.
- To identify a minimum set of STR markers for efficient donor-recipient identification.
Main Methods:
- Analyzed 15 STR loci in 90 donor-recipient pairs (60 discovery, 30 validation).
- Defined informative loci as those with 3 or 4 different alleles in combined recipient-donor genotypes.
- Utilized DNA extraction, STR-PCR, and fragment analysis.
Main Results:
- Informativity of STR loci ranged from 41.6% to 76.6%.
- The most informative loci identified were D2S1338, D21S11, D18S51, and FGA.
- A minimum panel of 8 STR markers provided at least 3 informative loci in 95% of cases.
Conclusions:
- The selected minimum STR panel offers an efficient method for detecting donor-recipient chimerism.
- This optimized panel can effectively quantitate chimerism post-transplantation.
- The findings support the clinical utility of this targeted STR approach.
