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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Imaging Studies II: Positron Emission Tomography and Scintigraphy01:25

Imaging Studies II: Positron Emission Tomography and Scintigraphy

Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...

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Related Experiment Video

Updated: May 26, 2026

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis
10:57

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

Published on: February 1, 2022

Raman scattering in pathology.

Zachary J Smith1, Thomas R Huser, Sebastian Wachsmann-Hogiu

  • 1Center for Biophotonic Science and Technology, University of California, Davis, USA.

Analytical Cellular Pathology (Amsterdam)
|December 14, 2011
PubMed
Summary
This summary is machine-generated.

Raman scattering, a technique analyzing light scattered by chemical bonds, shows promise for pathology. Recent developments highlight its potential in molecular sensing and disease detection for biomedical applications.

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A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer
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A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer

Published on: April 12, 2017

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Last Updated: May 26, 2026

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis
10:57

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

Published on: February 1, 2022

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer
07:52

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer

Published on: April 12, 2017

Area of Science:

  • Biomedical Optics
  • Chemical Spectroscopy
  • Pathology Imaging

Background:

  • Raman scattering provides molecular specificity by analyzing inelastic light scattering from chemical bonds.
  • It is applicable in both spectroscopy and imaging modes.
  • Biomedical applications are established, but pathology uses are emerging.

Purpose of the Study:

  • To review recent advancements in Raman scattering for pathology.
  • To cover relevant technologies, molecular sensing, and disease detection.
  • To highlight the potential of Raman scattering in biomedical pathology.

Main Methods:

  • Review of current literature on Raman scattering technologies.
  • Analysis of applications in molecular sensing.
  • Examination of cell and tissue characterization techniques.
  • Assessment of disease detection using Raman scattering.

Main Results:

  • Raman spectroscopy and imaging offer high molecular specificity.
  • Emerging applications demonstrate utility in molecular sensing and tissue analysis.
  • Potential for non-invasive disease detection is being explored.

Conclusions:

  • Raman scattering is a promising technology for pathology.
  • Further development is needed to fully realize its potential in disease diagnosis.
  • This technique offers new avenues for biomedical research and clinical applications.