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Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...

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Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
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Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

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Metal-organic frameworks for reverse-phase high-performance liquid chromatography.

Shan-Shan Liu1, Cheng-Xiong Yang, Shan-Wei Wang

  • 1State Key Laboratory of Medicinal Chemical Biology, and Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, China.

The Analyst
|December 14, 2011
PubMed
Summary
This summary is machine-generated.

Metal-organic framework MIL-53(Al) shows promise for high-performance liquid chromatography. It effectively separates diverse analytes, offering high resolution and reproducibility for analytical chemistry applications.

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Electrophoretic Crystallization of Ultrathin High-performance Metal-organic Framework Membranes
07:45

Electrophoretic Crystallization of Ultrathin High-performance Metal-organic Framework Membranes

Published on: August 16, 2018

Area of Science:

  • Analytical Chemistry
  • Materials Science

Background:

  • Metal-organic frameworks (MOFs) offer tunable properties for separation science.
  • MIL-53(Al) is a MOF with potential chromatographic applications.

Purpose of the Study:

  • To investigate the utility of MIL-53(Al) as a stationary phase in reverse-phase high-performance liquid chromatography (RP-HPLC).
  • To evaluate its separation performance for a broad spectrum of chemical analytes.

Main Methods:

  • Utilizing MIL-53(Al) as the stationary phase in a packed column for RP-HPLC.
  • Testing separation of non-polar, polar, acidic, and basic analytes.

Main Results:

  • Achieved high-resolution separations across a wide polarity range.
  • Demonstrated good selectivity, stability, and reproducibility.
  • MIL-53(Al) effectively separated diverse chemical compounds.

Conclusions:

  • MIL-53(Al) is a viable and effective stationary phase for RP-HPLC.
  • Its properties enable versatile chromatographic separations for various analytes.