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An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
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Aptamer optical biosensor without bio-breakage using upconversion nanoparticles as donors.

Kai Song1, Xianggui Kong, Xiaomin Liu

  • 1Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, P. R. China.

Chemical Communications (Cambridge, England)
|December 14, 2011
PubMed
Summary
This summary is machine-generated.

A novel aptamer-upconversion biosensor effectively detects ATP. This design prevents aptamer damage, ensuring high sensitivity and specificity for reliable ATP detection.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Optical biosensors are crucial for detecting biomolecules.
  • Aptamers offer high specificity for target recognition.
  • Upconversion nanoparticles (UCNPs) have unique photophysical properties.

Purpose of the Study:

  • To construct a Förster Resonance Energy Transfer (FRET)-based optical biosensor using an aptamer-upconversion nanoparticle conjugate.
  • To evaluate the biosensor's ability to detect adenosine triphosphate (ATP).
  • To address the issue of photosensitized aptamer damage in biosensor applications.

Main Methods:

  • Fabrication of an aptamer-upconversion nanoparticle conjugate.
  • Utilizing UCNPs as energy donors in a LRET system.
  • Assessing aptamer integrity and biosensor performance.
  • Measuring ATP concentration via the biosensor's response.

Main Results:

  • The constructed biosensor successfully detected ATP.
  • Employing UCNPs as donors eliminated photosensitized aptamer breakage and damage.
  • The biosensor demonstrated high specificity for ATP detection.
  • The biosensor exhibited excellent sensitivity in detecting ATP.

Conclusions:

  • The developed aptamer-upconversion conjugate biosensor is a robust platform for ATP detection.
  • The use of UCNPs effectively prevents aptamer photodamage, enhancing biosensor stability.
  • This approach offers a sensitive and specific method for optical detection of ATP.