Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved in...
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Benchtop High-MAS NMR for Paramagnetic Materials.

Molecules (Basel, Switzerland)·2026
Same author

Nanodiamond mediated delivery of pyridinium oxime antidotes to central nervous system for potential treatment of exposure to nerve agents.

Chemico-biological interactions·2025
Same author

Characterization of Photo-Cross-Linked Polyethylene Pipes for Geothermal Energy Storage.

ACS omega·2025
Same author

Valorizing Biopolyester Suberin: Modification of Cellulose Nanocrystals and Performance Assessment in 3D-Printed Biobased Acrylates.

ACS omega·2024
Same author

Production and Characterization of Melt-Spun Poly(3-hydroxybutyrate)/Poly(3-hydroxybutyrate-<i>co</i>-4-hydroxybutyrate) Blend Monofilaments.

ACS omega·2024
Same author

Solid-state NMR backbone chemical shift assignments of α-synuclein amyloid fibrils at fast MAS regime.

Biomolecular NMR assignments·2024

Related Experiment Video

Updated: May 26, 2026

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

New horizons for magic-angle spinning NMR.

Ago Samoson1, Tiit Tuherm, Jean Past

  • 1National Institute of Chemical Physics and Biophysics, Akadeemia Tee 23, Tallinn, Estonia, ago@kbfi.ee.

Topics in Current Chemistry
|December 14, 2011
PubMed
Summary

Recent advances in sample rotation technology have improved magic-angle-spinning Nuclear Magnetic Resonance (NMR) spectroscopy. This enables higher resolution solid-state (1)H spectroscopy and opens new avenues for studying physical phenomena.

More Related Videos

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning
07:33

Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning

Published on: April 15, 2010

Related Experiment Videos

Last Updated: May 26, 2026

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning
07:33

Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning

Published on: April 15, 2010

Area of Science:

  • Solid-state Nuclear Magnetic Resonance (NMR) spectroscopy
  • Advanced materials characterization
  • Physical chemistry

Background:

  • Magic-angle-spinning (MAS) NMR is a crucial technique for analyzing solid materials.
  • Limitations in rotation frequencies previously hindered high-resolution (1)H spectroscopy.
  • Exploring physical phenomena at cryogenic temperatures requires specialized NMR techniques.

Purpose of the Study:

  • To highlight the impact of recent sample rotation technology on MAS NMR.
  • To introduce novel spectroscopic methods enabled by these advancements.
  • To demonstrate the feasibility of high-resolution NMR at cryogenic temperatures.

Main Methods:

  • Implementation of advanced sample rotation technologies in MAS NMR.
  • Development of fast rotation sweep spectroscopy.
  • Utilizing cryogenic temperatures for enhanced NMR sensitivity.

Main Results:

  • Achieved rotation frequencies exceeding strong homonuclear spin interactions.
  • Enabled high-resolution solid-state (1)H spectroscopy.
  • Demonstrated feasibility of high-resolution NMR at cryogenic temperatures, providing significant sensitivity gains.

Conclusions:

  • New sample rotation technologies have revolutionized MAS NMR.
  • These advancements significantly improve spectral resolution and sensitivity.
  • Cryogenic MAS NMR opens new possibilities for studying diverse physical phenomena.