Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Production of Biopesticides

Production of Biopesticides

Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Characterisation of extracellular vesicles in baculovirus infection of <i>Spodoptera frugiperda</i> cells.

Journal of extracellular biology·2024

Same author

Development of humanised antibodies for Crimean-Congo Haemorrhagic fever virus: Comparison of hybridoma-based versus phage library techniques.

Journal of immunological methods·2022

Same author

ProPACC: Protocol for a Trial of Integrated Specialty Palliative Care for Critically Ill Older Adults.

Journal of pain and symptom management·2022

Same author

Tracing Baculovirus AcMNPV Infection Using a Real-Time Method Based on ANCHOR<sup>TM</sup> DNA Labeling Technology.

Viruses·2020

Same author

In cultured cells the baculovirus P10 protein forms two independent intracellular structures that play separate roles in occlusion body maturation and their release by nuclear disintegration.

PLoS pathogens·2019

Same author

Recent Developments in the Use of Baculovirus Expression Vectors.

Current issues in molecular biology·2019

Search research articles

Related Experiment Video

Updated: May 26, 2026

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Published on: August 20, 2018

High-throughput baculovirus expression in insect cells.

Richard B Hitchman¹, Robert D Possee, Linda A King

¹Oxford Expression Technologies Ltd, Oxford Brookes University, Oxford, UK. r.hitchman@oetltd.com

Methods in Molecular Biology (Clifton, N.J.)

|December 14, 2011

Summary

The flashBAC™ system simplifies baculovirus expression by enabling automated production of recombinant viruses. This novel vector eliminates the need for traditional selection methods, streamlining the process.

More Related Videos

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Published on: April 20, 2017

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

Published on: February 22, 2018

Related Experiment Videos

Last Updated: May 26, 2026

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Published on: August 20, 2018

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Published on: April 20, 2017

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

Published on: February 22, 2018

Area of Science:

Molecular Biology
Biotechnology
Virology

Background:

Traditional baculovirus expression systems face challenges in automation due to complex selection processes.
A key bottleneck in baculovirus expression is the separation of recombinant from parental viruses.
Existing methods often require laborious selection steps, hindering high-throughput applications.

Purpose of the Study:

To introduce a novel bacmid vector, flashBAC™, designed for simplified recombinant baculovirus production.
To demonstrate the utility of flashBAC™ in automated platforms for generating and testing multiple recombinant baculoviruses.
To overcome the limitations of traditional selection methods in baculovirus expression systems.

Main Methods:

Development of a replication-deficient bacmid vector (flashBAC™) that does not require selection pressure.
Utilizing homologous recombination in insect cells for gene of interest integration.
Replacing the bacterial replicon at the polyhedrin locus, restoring essential viral replication genes.

Main Results:

The flashBAC™ system enables direct production of recombinant baculovirus without parental virus separation.
Recombinant viruses, containing the gene of interest, are the only ones capable of replication.
Successful application of the flashBAC™ system for producing and expression testing multiple recombinant baculoviruses on automated platforms.

Conclusions:

The flashBAC™ system significantly simplifies the generation of recombinant baculoviruses.
This approach is highly suitable for automated platforms, increasing efficiency and throughput.
The elimination of selection pressure represents a major advancement in baculovirus expression technology.