Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical efficacy of mineralized collagen (MC) versus anorganic bovine bone (Bio-Oss) for immediate implant placement in esthetic area: a single-center retrospective study.

BMC oral health·2021
Same author

Corrigendum to: Enhancement of BMP-2 and VEGF Carried by Mineralized Collagen for Mandibular Bone Regeneration.

Regenerative biomaterials·2021
Same author

Influence of Surface Chemistry on Adhesion and Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells.

ACS biomaterials science & engineering·2021
Same author

Enhancement of BMP-2 and VEGF carried by mineralized collagen for mandibular bone regeneration.

Regenerative biomaterials·2020
Same author

Poly(methyl methacrylate) bone cement composited with mineralized collagen for osteoporotic vertebral compression fractures in extremely old patients.

Regenerative biomaterials·2020
Same author

Survival in Locally Advanced Pancreatic Cancer After Neoadjuvant Therapy and Surgical Resection.

Annals of surgery·2018
Same journal

RETRACTION: Effect of Surface Modification of Nanofibres with Glutamic Acid Peptide on Calcium Phosphate Nucleation and Osteogenic Differentiation of Marrow Stromal Cells.

Journal of tissue engineering and regenerative medicine·2026
Same journal

Mechanical Tuning of the Cell Microenvironment Using a Biomimetic Hydrogel System for Articular Cartilage Tissue Engineering.

Journal of tissue engineering and regenerative medicine·2026
Same journal

Pirfenidone Attenuates Fibrosis and Neovascularization in 3D Spheroid-Laden Hydrogel Culture.

Journal of tissue engineering and regenerative medicine·2026
Same journal

Cranial Defect Reconstruction With Custom 3D-Printed Hydroxyapatite Scaffolds Augmented With rhBMP-2 or Dipyridamole in a Nonhuman Primate Model.

Journal of tissue engineering and regenerative medicine·2026
Same journal

Collagen-Based Scaffolds for Meniscal Repair and Regeneration.

Journal of tissue engineering and regenerative medicine·2026
Same journal

Recent Advancements in the Generation and Application of Therapeutic Cell Populations for Lung Epithelial Repair.

Journal of tissue engineering and regenerative medicine·2026
See all related articles

Related Experiment Video

Updated: May 26, 2026

Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
07:35

Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures

Published on: March 7, 2015

Adipose stem cells controlled by surface chemistry.

Xi Liu1, Jin He, Shuming Zhang

  • 1State Key Laboratory of New Ceramics and Fine Processing, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.

Journal of Tissue Engineering and Regenerative Medicine
|December 14, 2011
PubMed
Summary
This summary is machine-generated.

Surface chemistry significantly influences human adipose stem cells (hASCs) differentiation. Specific functional groups promote osteogenic, chondrogenic, or adipogenic pathways, guiding tissue regeneration strategies.

More Related Videos

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
10:28

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

Published on: July 17, 2018

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats
10:50

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

Published on: April 7, 2023

Related Experiment Videos

Last Updated: May 26, 2026

Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
07:35

Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures

Published on: March 7, 2015

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
10:28

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

Published on: July 17, 2018

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats
10:50

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

Published on: April 7, 2023

Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Surface Chemistry

Background:

  • Human adipose stem cells (hASCs) hold great potential for tissue regeneration.
  • Controlling hASC behavior and differentiation is crucial for effective therapeutic applications.
  • Surface chemistry presents a tunable parameter to influence stem cell fate.

Purpose of the Study:

  • To investigate the impact of various surface chemistries on hASC morphology, growth, and differentiation.
  • To identify specific surface functional groups that promote osteogenic, chondrogenic, or adipogenic differentiation of hASCs.
  • To explore the potential of tailored surface chemistry in designing biomaterials for regenerative medicine.

Main Methods:

  • Utilized self-assembled monolayers of alkanethiolates on gold to create surfaces with diverse functional groups (e.g., NH₂, SH, COOH, Phenyl, Br, OH, CH₃).
  • Assessed hASC morphology and focal adhesion.
  • Evaluated cell growth using Cell Counting Kit-8 (CCK8) assay and cytoskeletal F-actin staining.
  • Determined mRNA expression of lineage-specific markers (ALP, OCN, collagen II, aggrecan, PPARγ, aP2) via RT-PCR.

Main Results:

  • Cell growth rate followed the order: NH₂ > SH > COOH > Phenyl > Br > OH > CH₃.
  • NH₂ surfaces promoted osteogenic differentiation.
  • Phenyl and SH surfaces enhanced chondrogenic differentiation, indicated by increased collagen II and aggrecan expression.
  • Br surfaces led to adipogenic differentiation, evidenced by elevated PPARγ and aP2 expression.

Conclusions:

  • Surface chemistry plays a critical role in modulating hASC differentiation pathways.
  • Specific surface functional groups can direct hASCs towards desired lineages (osteogenic, chondrogenic, adipogenic).
  • This study provides a chemical basis for designing advanced biomaterials for stem cell-based tissue regeneration.