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Related Concept Videos

mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...

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Updated: May 26, 2026

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

Sequence features of E. coli mRNAs affect their degradation.

Gal Lenz1, Adi Doron-Faigenboim, Eliora Z Ron

  • 1Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel.

Plos One
|December 14, 2011
PubMed
Summary

Bacterial messenger RNA (mRNA) stability is influenced by its sequence and structure. Key features like G+C content and folding energy, particularly at the 5' end, significantly impact mRNA half-life, affecting gene regulation.

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Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli
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Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
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Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

Area of Science:

  • Bacterial molecular biology
  • Gene regulation
  • Transcriptome analysis

Background:

  • Bacterial messenger RNA (mRNA) degradation is a crucial regulatory process for controlling protein levels.
  • While degradation mechanisms are understood, mRNA features influencing stability remain unclear.

Purpose of the Study:

  • To identify specific mRNA features that determine transcript stability in E. coli.
  • To correlate biophysical properties with experimental mRNA half-life data.

Main Methods:

  • Calculated G+C content, tRNA adaptation index (tAI), and folding energy for E. coli mRNAs.
  • Correlated these properties with measured transcript half-lives.
  • Utilized sliding window analysis to pinpoint critical regions.

Main Results:

  • Significant correlations were found between mRNA half-life and G+C content, tAI, and folding energy.
  • The 5' termini of mRNAs showed the strongest correlation with half-life for G+C content and folding energy.
  • Each parameter independently contributes to mRNA stability.

Conclusions:

  • mRNA sequence and structure, especially at the 5' end, are key determinants of bacterial mRNA stability.
  • Newly acquired genes in E. coli exhibit higher mRNA stability due to distinct nucleotide composition, potentially aiding evolutionary integration.