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Updated: May 26, 2026

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis
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Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis

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A guided mode resonance aptasensor for thrombin detection.

Sheng-Fu Lin1, Ting-Jou Ding, Jen-Tsai Liu

  • 1Department of Optics and Photonics, National Central University, Jhongli 32001, Taiwan. 982406004@cc.ncu.edu.tw

Sensors (Basel, Switzerland)
|December 14, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a novel aptasensor using a guided mode resonance (GMR) device for detecting thrombin. The developed biosensor offers sensitive, label-free detection, paving the way for real-time diagnostics.

Keywords:
affinity constantaptamerguided mode resonanceoptical biosensorthrombin

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Area of Science:

  • Biosensing
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Aptamers are increasingly used in biosensing applications.
  • Guided Mode Resonance (GMR) devices offer unique sensing capabilities.
  • Combining aptamers with GMR devices for biosensing is an unexplored area.

Purpose of the Study:

  • To develop and characterize a novel aptasensor by integrating aptamers with a home-built GMR device.
  • To demonstrate the feasibility of using this GMR aptasensor for label-free thrombin detection.
  • To evaluate the sensitivity and binding kinetics of the developed aptasensor.

Main Methods:

  • Immobilization of a 29-mer thrombin aptamer onto a GMR device surface.
  • Utilizing the GMR device for real-time, label-free detection of thrombin.
  • Characterization of sensor performance, including sensitivity and limit of detection (LOD).

Main Results:

  • Achieved a sensitivity of 0.04 nm/μM for thrombin detection within the 0.25–1 μM concentration range.
  • Determined a limit of detection (LOD) of 0.19 μM.
  • Measured a binding affinity constant (Ka) in the range of 10(6) M(-1), indicating efficient thrombin binding.

Conclusions:

  • The GMR aptasensor demonstrates high sensitivity for real-time, label-free thrombin detection.
  • This novel approach provides kinetic information crucial for understanding thrombin binding.
  • The developed GMR aptasensor shows significant potential for diagnostic applications.