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Related Concept Videos

NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
NMR Spectroscopy Of Amines01:19

NMR Spectroscopy Of Amines

In proton NMR spectroscopy, primary amines and secondary amines showcase their N–H protons as a broad signal in the chemical shift range between δ 0.5 and 5 ppm. The exact position in this range depends on several factors, including sample concentration, hydrogen bonding, and the type of solvent used. Since amine protons undergo fast proton exchange in solution, the protons are labile and therefore do not participate in any splitting with adjacent protons. Thus, the observed peak is broad and...
NMR Spectroscopy of Aromatic Compounds01:14

NMR Spectroscopy of Aromatic Compounds

Aromatic compounds can be identified or analyzed using proton NMR and carbon‐13 NMR. Typically, aromatic hydrogens or hydrogens directly bonded to the aromatic rings are strongly deshielded by the aromatic ring current. Therefore, they absorb in the range of 6.5–8.0 ppm in proton NMR spectra. For instance, aromatic hydrogens directly bonded to the benzene ring absorb at 7.3 ppm. However, aromatic hydrogens of larger rings absorb farther upfield or downfield than the ideal range. Consider...
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
Nuclear Magnetic Resonance (NMR): Overview01:07

Nuclear Magnetic Resonance (NMR): Overview

Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...

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Related Experiment Video

Updated: May 26, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

ARIA for solution and solid-state NMR.

Benjamin Bardiaux1, Thérèse Malliavin, Michael Nilges

  • 1NMR-supported Structural Biology, Leibnitz-Institutfür Molekulare Pharmakologie (FMP), Berlin, Germany. bardiaux@fmp-berlin.de

Methods in Molecular Biology (Clifton, N.J.)
|December 15, 2011
PubMed
Summary
This summary is machine-generated.

The Ambiguous Restraints for Iterative Assignment (ARIA) program automates biomolecular structure determination using Nuclear Magnetic Resonance (NMR) data. ARIA 2.3 enhances this process for both solution and solid-state NMR, improving accuracy and efficiency.

Related Experiment Videos

Last Updated: May 26, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Area of Science:

  • Biochemistry and Structural Biology
  • Computational Chemistry
  • Spectroscopy

Background:

  • Determining biomolecular 3D structure via NMR requires distance information from spectra.
  • Interpreting NMR spectra is crucial for accurate structure determination.
  • Existing methods often require manual intervention for cross-peak assignment.

Purpose of the Study:

  • To present the Ambiguous Restraints for Iterative Assignment (ARIA) program for automated NMR structure determination.
  • To detail the concepts, procedures, and strategy of ARIA 2.3 for solution and solid-state NMR.
  • To highlight recent advancements in ARIA, including CCPN integration and enhanced restraint potentials.

Main Methods:

  • Automated cross-peak assignment using Nuclear Overhauser Effect (NOE) data.
  • Structure determination from solution and solid-state NMR experiments.
  • Interpretation of magic-angle spinning (MAS) solid-state NMR data.

Main Results:

  • ARIA program provides automated assignment and structure determination from NMR data.
  • ARIA 2.3 extends capabilities to solid-state NMR (MAS).
  • Recent developments include enhanced integration with CCPN and improved restraint potentials.

Conclusions:

  • ARIA program facilitates efficient and automated biomolecular structure determination using NMR.
  • ARIA 2.3 represents a significant advancement for both solution and solid-state NMR structure analysis.
  • The program's automated features streamline the interpretation of complex NMR spectra.