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Updated: May 26, 2026

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
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Published on: March 1, 2013

Transgene delivery using poly(amino ether)-gold nanorod assemblies.

James Ramos1, Kaushal Rege

  • 1School of Biological and Health Systems Engineering, Arizona State University, Tempe, Arizona 85287-6106, USA.

Biotechnology and Bioengineering
|December 16, 2011
PubMed
Summary

New gold nanorod (GNR) formulations show enhanced gene delivery to cancer cells. Poly(amino ether)-functionalized GNRs (PAE-GNRs) offer improved transgene expression and reduced toxicity compared to existing methods.

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Last Updated: May 26, 2026

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Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods
10:46

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods

Published on: May 2, 2016

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Materials Science

Background:

  • Gold nanorods (GNRs) are versatile nanomaterials with applications in diagnostics and therapeutics.
  • Effective gene delivery systems are crucial for treating diseases like cancer.
  • Developing stable and efficient GNR-based delivery platforms is an ongoing challenge.

Purpose of the Study:

  • To develop and characterize poly(amino ether)-functionalized gold nanorods (PAE-GNRs) for enhanced gene delivery.
  • To compare the efficacy and safety of PAE-GNRs against established gene delivery systems.
  • To investigate the influence of polyelectrolyte chemistry and zeta-potential on gene delivery performance.

Main Methods:

  • Layer-by-layer deposition was used to create PAE-GNR assemblies.
  • Plasmid DNA binding via electrostatic interactions was confirmed.
  • In vitro transfection of prostate cancer cells with PAE-GNRs carrying plasmid DNA was performed.
  • Cytotoxicity and transgene expression levels were quantified and compared.

Main Results:

  • PAE-GNR assemblies exhibited long-term colloidal stability and effective plasmid DNA binding.
  • PAE-GNRs formulated with 1,4C-1,4Bis demonstrated significantly higher transgene expression than PEI25k-GNRs.
  • PAE-GNRs showed lower cytotoxicity compared to PEI25k-GNRs.
  • Polyelectrolyte chemistry and zeta-potential were identified as key factors influencing gene delivery efficacy.

Conclusions:

  • Stable and effective PAE-GNR assemblies represent a promising platform for transgene delivery.
  • PAE-GNRs offer superior gene delivery performance and safety profiles compared to PEI25k-GNRs.
  • PAE-GNRs hold potential as a theranostic platform for combined therapies and imaging.