Expression of ecto-ATPase NTPDase2 in human dental pulp
1Division of General Dentistry, University of Rochester Eastman Institute for Oral Health, 625 Elmwood Avenue, Rochester, NY 14620, USA.
Journal of Dental Research
|December 17, 2011
Summary
This study found nucleoside triphosphate diphosphohydrolases (NTPDases) in human dental pulp, specifically NTPDase2, which helps break down adenosine triphosphate (ATP). This suggests ATP signaling plays a role in dental pain and sensitivity.
Area of Science:
- Oral Biology
- Neuroscience
- Biochemistry
Background:
- Dental nerve fibers utilize adenosine triphosphate (ATP) receptors, indicating ATP's role in dental pain signaling.
- Extracellular ATP hydrolysis is primarily mediated by nucleoside triphosphate diphosphohydrolases (NTPDases).
Purpose of the Study:
- To investigate the presence and localization of NTPDases in human dental pulp.
- To explore the potential role of ATP signaling in dentin sensitivity and dental pain.
Main Methods:
- Immunohistochemistry and immunofluorescence techniques were employed.
- Localization of NTPDase2 and connexin43 in human dental pulp tissue was examined.
Main Results:
- NTPDase2 was identified in pulpal nerve bundles, Raschkow's plexus, and the odontoblast layer.
- NTPDase2 was found in pulpal Schwann cells and odontoblasts, often near connexin43.
- Evidence suggests an ATP release and degradation system exists in human dental pulp.
Conclusions:
- The presence of NTPDase2 supports the involvement of ATP signaling in dental nociception.
- Findings indicate a functional apparatus for ATP signaling in human dental pulp, contributing to dentin sensitivity and pain perception.
