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Related Experiment Video

Updated: May 26, 2026

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

A lateral flow protein microarray for rapid and sensitive antibody assays.

Jesper Gantelius¹, Tarek Bass, Ronald Sjöberg

¹Division of Nanobiotechnology, School of Biotechnology, KTH-Royal Institute of Technology, Albanova University Center, 106 91 Stockholm, Sweden; jesper.gantelius@biotech.kth.se.

International Journal of Molecular Sciences

|December 17, 2011

Summary

A new, easy-to-use lateral flow protein microarray enables sensitive detection of antigen-specific antibodies in ten minutes. This portable device offers accurate results comparable to lab-based assays, ideal for mobile settings.

Keywords:

lateral flow point of care protein microarray

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Area of Science:

Biotechnology
Assay Development
Point-of-Care Diagnostics

Background:

Protein microarrays are valuable for biomarker detection but typically confined to laboratory settings.
Existing methods lack portability and speed for widespread clinical application.

Purpose of the Study:

To develop a novel, user-friendly lateral flow protein microarray for rapid, multiplexed antibody detection.
To enable sensitive biomarker quantification outside traditional laboratory environments.

Main Methods:

A 384-plexed lateral flow protein microarray device was engineered for ease of use.
Assays utilized gold nanobeads for signal development, with results read by cellphone cameras or scanners.
Performance was validated against established glass microarray assays.

Main Results:

Achieved sensitive detection of antigen-specific antibodies (<30 ng/mL) within a 10-minute assay time.
Demonstrated excellent accuracy (98% AUC) for 26 antibody targets compared to glass microarrays.
Enabled results to be read using portable imaging devices.

Conclusions:

The developed lateral flow protein microarray offers a convenient and cost-efficient solution for antibody quality control.
This platform supports multiplexed, affinity-based assays in resource-limited or mobile healthcare settings.
Facilitates broader accessibility of advanced biomarker detection technologies.