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Development of a screening method for biogenic amine producing Bacillus spp.
1Department of Food and Nutrition, Kimchi Research Center, Chosun University, Gwangju 501–759, Republic of Korea.
International Journal of Food Microbiology
|December 20, 2011
Summary
A new method effectively detects biogenic amine (BA)-producing Bacillus strains, overcoming limitations of pH-based methods. This reliable technique enhances accuracy and reduces false positives in microbial screening.
Area of Science:
- Microbiology
- Biochemistry
Background:
- Current biogenic amine (BA) detection relies on pH indicators, unsuitable for alkaline bacteria like Bacillus.
- This limits screening capabilities for BA-producing Bacillus species.
Purpose of the Study:
- To develop a rapid, reliable method for detecting BA-producing Bacillus strains.
- To improve accuracy and reduce false positives in microbial screening.
Main Methods:
- Development of novel plate and broth methods for BA detection in Bacillus.
- Utilized glycerol metabolism test to screen out false positives.
- Confirmed results with high-performance liquid chromatography (HPLC).
Main Results:
- The plate method provided positive results within 24 hours.
- The broth method offered enhanced precision, quantitation, and reduced false negatives.
- Five of twelve strains were identified as BA-positive, with one confirmed false positive via glycerol metabolism test.
Conclusions:
- The new broth method is accurate and reliable for detecting BA-producing Bacillus.
- This method overcomes limitations of traditional pH-based assays.
- The glycerol metabolism test effectively eliminates false positives.
