Electrophilic levuglandin E2-protein adducts bind glycine: a model for protein crosslinking

Prevention by glycine of protein crosslinking which accompanies binding of levuglandin E2 (LGE2) is shown to involve binding of glycine with the protein-LGE2 adduct. With ovalbumin, the LGE2 adduct initially binds nearly 2 equivalents of glycine, but the capacity to bind glycine decreases with time reflecting a competition, inter alia, with crosslinking.