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Updated: May 26, 2026
A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos
Published on: November 8, 2014
Chemical informatics and target identification in a zebrafish phenotypic screen
Christian Laggner1, David Kokel, Vincent Setola
1Department of Pharmaceutical Chemistry, University of California, San Francisco, California, USA.
None:
Target identification is a core challenge in chemical genetics. Here we use chemical similarity to computationally predict the targets of 586 compounds that were active in a zebrafish behavioral assay. Among 20 predictions tested, 11 compounds had activities ranging from 1 nM to 10,000 nM on the predicted targets. The roles of two of these targets were tested in the original zebrafish phenotype. Prediction of targets from chemotype is rapid and may be generally applicable.
