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Competitive erythroimmunoassay for detecting Clostridium perfringens type A enterotoxin in stool specimens
Y Germani1, M R Popoff, E Bégaud
1Institut Pasteur de Nouméa, Laboratoire des Bactéries entéropathogénes, Nouméa.
Research in Microbiology
|June 1, 1990
Abstract:
A competitive erythroimmunoassay (ERIA) is described for Clostridium perfringens enterotoxin (CPE) detection in stools. This technique uses sheep red blood cells sensitized by CPE and an anti-CPE-antibody-coated plate in which the results are read by eye. ERIA is simple, rapid, economic and more sensitive (2 ng/ml) than the enzyme-linked immunosorbent assay used for evaluation. ERIA is suitable for CPE detection in stool samples protected with phenylmethylsulphonylfluoride.