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Updated: May 26, 2026

In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy
Published on: September 6, 2024
Analysis of changes in nucleosome conformation using fluorescence resonance energy transfer
Tina Shahian1, Geeta J Narlikar
1Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA.
Abstract:
ATP-dependent nucleosome-remodeling motors use the energy of ATP to alter the accessibility of the underlying DNA. Understanding how these motors alter nucleosome structure can be aided by following changes in histone-DNA contacts in real time. Here, we describe a fluorescence resonance energy transfer-based approach that enables visualization of such changes.
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