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Updated: May 26, 2026

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Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
Published on: January 3, 2014
Identification of HR-inducing cDNAs from plant pathogens via a Gateway(®)-compatible binary Potato virus X-expression
1Laboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands. Peter.vanesse@wur.nl
Methods in Molecular Biology (Clifton, N.J.)
|December 21, 2011
Abstract:
Identification of pathogen effectors that elicit a hypersensitive response (HR) in resistant plant hosts is essential to study disease resistance. In this method, it is described how to generate a cDNA library, how to transfer the library into a binary PVX-expression vector, and finally how to set up a high-throughput screen for HR-inducing cDNAs from plant pathogens.
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