Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Queen and the Dark Twin: Heme, Protoporphyrin IX, and State Transitions in Liver Metabolism.

Molecules (Basel, Switzerland)·2026
Same author

Russian Dolls of Heme Metabolism in Malaria-Infected Red Blood Cells: Nested Vulnerabilities and Therapeutic Opportunities.

Pathogens (Basel, Switzerland)·2026
Same author

Targeting Infected Host Cell Heme Metabolism to Kill Malaria Parasites.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Spatial Transcriptomics Reveals Distinct Architectures but Shared Vulnerabilities in Primary and Metastatic Liver Tumors.

Cancers·2025
Same author

Genetic Evidence of <i>Yersinia pestis</i> from the First Pandemic.

Genes·2025
Same author

Ancient Origins and Global Diversity of Plague: Genomic Evidence for Deep Eurasian Reservoirs and Recurrent Emergence.

Pathogens (Basel, Switzerland)·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 26, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Massively parallel sequencing technology in pathogenic microbes.

Sucheta Tripathy1, Rays H Y Jiang

  • 1Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA. sutripa@vbi.vt.edu

Methods in Molecular Biology (Clifton, N.J.)
|December 21, 2011
PubMed
Summary
This summary is machine-generated.

Next-Generation Sequencing (NGS) offers a powerful, cost-effective, and accurate method for digital gene expression analysis. This technology is transforming our understanding of host-pathogen interactions in fungi and oomycetes.

More Related Videos

Amplicon Sequencing using the Long-Read Sequencing Technologies
08:57

Amplicon Sequencing using the Long-Read Sequencing Technologies

Published on: August 29, 2025

Related Experiment Videos

Last Updated: May 26, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

Amplicon Sequencing using the Long-Read Sequencing Technologies
08:57

Amplicon Sequencing using the Long-Read Sequencing Technologies

Published on: August 29, 2025

Area of Science:

  • Genomics
  • Transcriptomics
  • Bioinformatics

Background:

  • Next-Generation Sequencing (NGS) has rapidly advanced genomics, particularly in transcriptome analysis.
  • Digital gene expression analysis using NGS is replacing older microarray technologies due to superior cost-effectiveness, reproducibility, accuracy, and speed.
  • Significant advancements in statistical methods and software for NGS data analysis and visualization have occurred recently.

Purpose of the Study:

  • To describe the core technology of new sequencing platforms.
  • To outline the methodology for analyzing Next-Generation Sequencing data.
  • To explore diverse applications of NGS in biological research, with a focus on host-pathogen interactions.

Main Methods:

  • Utilizing Next-Generation Sequencing (NGS) for high-throughput sequencing.
  • Applying advanced statistical methods for the analysis and visualization of large NGS datasets.
  • Focusing on data from pathogenic fungi and oomycetes.

Main Results:

  • NGS enables digital expression analysis, offering advantages over traditional analog methods.
  • Analysis of NGS data is revealing new insights into host-pathogen interactions.
  • Discoveries include novel transcripts, splice variants, mutations, regulatory elements, and epigenetic controls.

Conclusions:

  • Next-Generation Sequencing is revolutionizing genomics and transcriptome analysis.
  • The application of NGS to pathogenic fungi and oomycetes is shifting our understanding of host-pathogen dynamics.
  • Continued development in NGS technology and analytical tools promises further breakthroughs.