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Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Related Experiment Video

Updated: May 26, 2026

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Published on: September 30, 2016

Identifying genes in Fusarium verticillioides through forward and reverse genetics.

J B Ridenour¹, R L Hirsch, B H Bluhm

¹Division of Agriculture, Department of Plant Pathology, University of Arkansas, Fayetteville, AR, USA.

Methods in Molecular Biology (Clifton, N.J.)

|December 21, 2011

Summary

This study details methods for identifying genes in plant pathogenic fungi. Researchers used forward and reverse genetics in Fusarium verticillioides to understand pathogenesis.

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Published on: April 13, 2012

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

Published on: August 23, 2013

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Last Updated: May 26, 2026

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Published on: September 30, 2016

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Published on: April 13, 2012

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

Published on: August 23, 2013

Area of Science:

Molecular Plant Pathology
Fungal Genetics
Genomics

Background:

Sequenced genomes have advanced plant pathology, but genetic regulatory networks in pathogenesis are poorly understood.
Filamentous fungi present complex biological processes that require robust genetic identification methods.

Purpose of the Study:

To describe protocols for identifying genes involved in fungal pathogenesis using forward and reverse genetics.
To utilize Fusarium verticillioides as a model organism for developing and demonstrating these genetic techniques.

Main Methods:

Generation of insertional mutants using Restriction Enzyme Mediated Integration (REMI).
High-throughput screening of mutants for amylolysis defects.
Identification of genomic lesions in REMI mutants via genome-walker PCR.
Disruption of candidate genes in wild-type strains using a split-marker strategy for phenotype confirmation.

Main Results:

Established protocols for creating and screening insertional mutants in Fusarium verticillioides.
Successfully identified candidate genes associated with amylolysis defects.
Validated gene function through reverse genetics using a split-marker approach.

Conclusions:

The described protocols provide a comprehensive framework for gene discovery in plant pathogenic fungi.
This approach enhances the understanding of genetic regulatory networks critical for fungal pathogenesis.
The methods are applicable to a wide range of filamentous fungi for studying diverse biological processes.