Related Experiment Video
Updated: May 26, 2026

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
Microbiological analysis of gingivitis in pediatric patients under orthodontic treatment
C Cardoso-Silva1, E Barbería, J A Ramos Atance
1Faculty of Dental Medicine, University of Porto, Portugal. cristinalsilva@mail.telepac.pt
Aim:
The aim of this study was to determine the relationship between gingival inflammation and changes in bacteria of the gingival sulcus in children in orthodontic treatment with brackets.
Study Design:
this prospective study assessed gingival and plaque index of two groups: children with brackets (Group 1) and without brackets (Group 2). The sample was selected from patients treated at the Faculty of Dentistry, Complutense University of Madrid, Spain. Microbiological assessment was performed in every child and all data were statistically analysed.
Results:
Group 1 showed significantly higher microbiological values and the difference was greater in lower teeth. Comparing the total plaque percentage, it was significantly higher in Group 1.
Statistics:
there was no significant correlation between gingival and plaque indexes in any group. No significant correlation was found between plaque index and bacteria.
Conclusion:
Children using brackets showed significantly higher gingival and plaque indices than children without brackets. No direct relationship was found between the increase in gingival and plaque indices and the presence and quantity of bacteria; therefore it was not possible to identify specific bacteria as responsible for the high gingival index in patients with brackets.
Related Concept Videos
The Oral Microbiota
Development of the Oral Microbiota
