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Published on: March 6, 2019
Indirect inactivation of tyrosinase in its action on tyrosine
Jose Luis Muñoz-Muñoz1, Francisco Garcia-Molina, Jose Ramon Acosta-Motos
1GENZ: Grupo de Investigación Enzimología, Departamento de Bioquímica y Biología Molecular-A, Universidad de Murcia, Espinardo, Murcia, Spain.
Abstract:
Under aerobic conditions, tyrosinase is inactivated by dopa as a result of suicide inactivation, and, under anaerobic conditions, as a result of irreversible inactivation. However, tyrosine protects the enzyme from being inactivated by dopa under anaerobic conditions. This paper describes how under aerobic conditions the enzyme acting on tyrosine is not directly inactivated but undergoes a process of indirect suicide inactivation provoked by reaction with the o-diphenol originated from the evolution of o-dopaquinone and accumulated in the reaction medium.
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