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Related Experiment Video

Updated: May 26, 2026

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

Single-cell protein analysis.

Meiye Wu1, Anup K Singh

  • 1Department of Bioengineering and Biotechnology, Sandia National Laboratory, Livermore, CA 94550, United States.

Current Opinion in Biotechnology
|December 23, 2011
PubMed
Summary
This summary is machine-generated.

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Single-cell protein analysis is now possible thanks to new technologies. This review covers recent advances in measuring proteins in single cells, excluding imaging methods, and their biological research significance.

Area of Science:

  • Cellular biology
  • Molecular biology
  • Biotechnology

Background:

  • Cellular heterogeneity is a recognized phenomenon.
  • Single-cell analysis tools are recent advancements.
  • Genomic analysis in single cells is aided by amplification techniques like PCR.

Purpose of the Study:

  • To review recent developments in single-cell protein analysis.
  • To discuss the significance of these techniques in biological research.
  • To focus on non-imaging-based protein analysis methods.

Main Methods:

  • Multi-parameter flow cytometry
  • Microfluidics
  • Other advanced techniques for protein measurement in single cells

Main Results:

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Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
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Published on: February 1, 2022

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Last Updated: May 26, 2026

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
06:13

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
09:20

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Published on: February 1, 2022

  • Recent technological advances enable measurement of a wide variety of proteins in single cells.
  • Progress has been made in overcoming challenges in single-cell protein analysis.
  • Several techniques are now available for comprehensive protein profiling.

Conclusions:

  • Single-cell protein analysis is a rapidly advancing field.
  • These techniques are crucial for understanding cellular heterogeneity.
  • The reviewed methods hold significant promise for future biological research.