Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Novel Peptide Multimer for Enhanced Imaging and Multivalent Detection of Hepatocellular Carcinoma.

Sensors and actuators. B, Chemical·2026
Same author

Development of a Telehealth-Enabled Portable Optical Endomicroscopy System with Targeted Peptides: A Preclinical Feasibility Study for Cervical Cancer Detection.

Cancers·2026
Same author

Compact, scan-pattern-switchable 2D piezoelectric MEMS mirror with 1D addressable scanning.

Journal of microelectromechanical systems : a joint IEEE and ASME publication on microstructures, microactuators, microsensors, and microsystems·2026
Same author

Identification of tumor initiating cells and early marker genes in histologically normal colonic mucosa that lead to neoplastic transformation.

Neoplasia (New York, N.Y.)·2026
Same author

Mammogram Analysis with YOLO Models on an Affordable Embedded System.

Cancers·2026
Same author

Identification of tumor initiating cells and early marker genes in normal colonic epithelium that lead to neoplastic transformation.

Research square·2025
Same journal

Uniform 2D Target Generation via Inverse-designed Metasurfaces.

IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society·2026
Same journal

Comprehensive Optimization of Interferometric Diffusing Wave Spectroscopy (iDWS).

IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society·2026
Same journal

Unified Vibrational and Multiphoton Label-Free Nonlinear Microscopy for Simultaneous Chemical and Structural Imaging.

IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society·2026
Same journal

Corrections to "Nonlinear Imaging Histopathology: A Pipeline to Correlate Gold-Standard Hematoxylin and Eosin Staining With Modern Nonlinear Microscopy".

IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society·2026
Same journal

Label-Free Optical Investigation of Structural and Thermal Changes in Fat and Lean Beef under Frozen Storage.

IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society·2026
Same journal

Non-contact optical spectroscopy for metabolic and vascular characterizations of orthotopic tongue cancer models <i>in vivo</i>.

IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society·2026
See all related articles

Related Experiment Video

Updated: May 26, 2026

Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

MEMS-Based Dual Axes Confocal Microendoscopy.

Wibool Piyawattanametha1, Thomas D Wang

  • 1National Electronics and Computer Technology Center, Pathumthani 12120, Thailand; Departments of Applied Physics, Biology, Electrical Engineering, Microbiology & Immunology, and Pediatrics, Stanford University, CA 94305 USA ( wibool@gmail.com ).

IEEE Journal of Selected Topics in Quantum Electronics : a Publication of the IEEE Lasers and Electro-Optics Society
|December 23, 2011
PubMed
Summary
This summary is machine-generated.

We developed a miniature near-infrared microscope with dual axes confocal architecture for real-time in vivo imaging. This advanced microscopy offers sub-cellular resolution and deep tissue penetration, enabling precise diagnostics and therapy monitoring.

More Related Videos

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
10:35

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique
06:31

Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique

Published on: January 10, 2013

Related Experiment Videos

Last Updated: May 26, 2026

Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
10:35

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique
06:31

Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique

Published on: January 10, 2013

Area of Science:

  • Biomedical optics
  • Microscopy engineering

Background:

  • Confocal microscopy offers high resolution but often lacks scalability and real-time in vivo capabilities.
  • Miniaturization of advanced optical instruments is crucial for minimally invasive diagnostics.

Purpose of the Study:

  • To demonstrate a novel miniature near-infrared microscope with dual axes confocal architecture.
  • To achieve sub-cellular resolution with deep tissue penetration and a large field of view for in vivo imaging.
  • To develop an endoscope-compatible version for digestive tract imaging.

Main Methods:

  • Utilized a novel dual axes confocal architecture.
  • Implemented post-objective scanning for scalability.
  • Employed a MEMS mirror for real-time (>4 Hz) in vivo imaging.
  • Developed an endoscope-compatible probe.

Main Results:

  • Achieved sub-cellular resolution.
  • Demonstrated deep tissue penetration.
  • Enabled large field of view imaging.
  • Performed real-time in vivo imaging at >4 Hz.
  • Successfully imaged digestive tract epithelium.

Conclusions:

  • The developed miniature microscope provides a versatile platform for high-resolution in vivo imaging.
  • The endoscope-compatible version facilitates targeted tissue biopsy guidance and therapy monitoring in the digestive tract.