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Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Updated: May 26, 2026

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Published on: October 25, 2019

Derivatization methods for quantitative bioanalysis by LC-MS/MS.

Pan Deng¹, Yan Zhan, Xiaoyan Chen

¹Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Road, Shanghai 201203, China.

|December 24, 2011

Summary

This summary is machine-generated.

Chemical derivatization enhances quantitative analysis of challenging compounds using liquid chromatography-mass spectrometry (LC-MS/MS). This method improves ionization, separation, and stability for diverse molecules in biological samples.

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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Published on: May 20, 2013

Area of Science:

Analytical Chemistry
Biochemistry
Pharmacology

Background:

Liquid chromatography coupled with atmospheric pressure ionization mass spectrometry (LC-MS/MS) is crucial for quantitative bioanalysis, particularly for nonvolatile and polar compounds.
Challenges such as weak ionization, poor chromatographic retention, and analyte instability often impede the development of robust LC-MS/MS methods.
Chemical derivatization is a widely adopted strategy to overcome these limitations.

Purpose of the Study:

To provide a comprehensive overview of chemical derivatization techniques employed in quantitative LC-MS/MS analysis.
To highlight the application of derivatization for improving the analysis of specific compound classes in biological matrices.

Main Methods:

Review of chemical derivatization strategies applied to nine distinct classes of molecules.
Focus on methods enhancing ionization efficiency, chromatographic separation, and analyte stability for LC-MS/MS.
Analysis of diverse analytes including aldehydes, amino acids, bisphosphonate drugs, carbohydrates, carboxylic acids, nucleosides, steroids, thiols, and vitamin D metabolites.

Main Results:

Chemical derivatization effectively addresses challenges in quantitative LC-MS/MS analysis.
Demonstrated improvements in ionization, chromatographic behavior, and stability across various compound classes.
Successful application in the analysis of complex biological samples.

Conclusions:

Chemical derivatization is an indispensable tool for advancing quantitative LC-MS/MS bioanalysis.
It enables the reliable quantification of previously challenging analytes.
This approach significantly expands the scope and accuracy of mass spectrometry-based bioanalytical methods.