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Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Lensless multispectral digital in-line holographic microscope.

James P Ryle¹, Susan McDonnell, John T Sheridan

¹University College Dublin, Communications and Optoelectronic Research Centre, Belfield, Dublin D4, Ireland.

Journal of Biomedical Optics

|December 24, 2011

Summary

A new compact multispectral digital in-line holographic microscope (DIHM) captures detailed images of cells and materials. This holographic microscopy technique enables quantitative analysis and phase profiling, offering an alternative to existing methods.

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Area of Science:

* Optics and Photonics
* Biomedical Imaging
* Microscopy

Background:

* Holography offers a powerful method for 3D imaging and quantitative phase retrieval.
* Digital holographic microscopy (DHM) has advanced imaging capabilities, but limitations exist in current systems.
* Multispectral imaging provides richer information by capturing data across different wavelengths.

Purpose of the Study:

* To develop and validate a compact multispectral digital in-line holographic microscope (DIHM).
* To emulate Gabor's original holographic principle for enhanced imaging.
* To assess the DIHM's performance in quantitative analysis and phase profiling of biological and material samples.

Main Methods:

* Construction of a compact multispectral digital in-line holographic microscope.
* Utilizing light sources with varying spatial coherence (laser, LED).
* Numerical processing of captured holographic images for analysis and visualization.

Main Results:

* Successful holographic image capture and processing of diverse samples, including optical fiber, microspheres, and cancer cells.
* Quantitative estimation of cell locations and confluence percentage.
* Obtained phase profiles of weakly scattering cells, comparable to off-axis DHM.

Conclusions:

* The developed DIHM is a viable tool for high-resolution imaging and quantitative analysis.
* The system demonstrates potential for biological cell studies, including confluence monitoring.
* DIHM offers a promising alternative for phase contrast imaging of delicate biological samples.