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Making every cell like HeLa a giant step for cell culture

Seema Agarwal1, David L Rimm

  • 1Department of Pathology, Yale University School of Medicine, New Haven, CT 06520-8023, USA.

The American Journal of Pathology
|December 24, 2011
PubMed
Summary

No abstract available in PubMed .

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Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

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