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Updated: May 26, 2026

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment
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Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment

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Three-dimensional positioning of optically trapped nanoparticles.

Takayuki Higuchi1, Quang Duc Pham, Satoshi Hasegawa

  • 1Center for Optical Research and Education (CORE), Utsunomiya University, 7-1-2 Yoto, Utsunomiya 321-8585, Japan.

Applied Optics
|December 24, 2011
PubMed
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We developed a 3D holographic microscope to precisely measure nanometer spheres trapped by optical tweezers. This method accurately tracks particle position and analyzes Brownian motion, aiding optical trapping studies.

Area of Science:

  • Optics and Photonics
  • Nanotechnology
  • Biophysics

Background:

  • Optical tweezers are crucial for manipulating microscopic objects.
  • Accurate 3D positioning of nanoparticles is essential for various scientific applications.
  • Digital holographic microscopy offers high-resolution imaging capabilities.

Purpose of the Study:

  • To demonstrate 3D measurement of nanometer-sized spheres using digital holographic microscopy.
  • To assess the positioning resolution of the developed holographic microscope.
  • To investigate Brownian motion changes in response to optical trapping parameters.

Main Methods:

  • Utilized an in-line digital holographic microscope with a green LED light source.
  • Employed optical tweezers to immobilize a 200 nm polystyrene sphere in water.

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A Protocol for Real-time 3D Single Particle Tracking
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Optical Trapping of Nanoparticles
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Published on: January 15, 2013

Related Experiment Videos

Last Updated: May 26, 2026

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment
09:13

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment

Published on: April 4, 2017

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

  • Measured 3D positions and analyzed Brownian motion by varying laser power.

    • Main Results:

    • Achieved positioning resolutions of 3.2 nm (transverse) and 3.4 nm (axial) for a fixed 200 nm sphere.
    • Successfully detected 3D positions of the sphere held by optical tweezers.
    • Observed and quantified changes in Brownian motion with varying optical trapping power.

    Conclusions:

    • The developed holographic microscope enables precise 3D measurement of nanometer spheres.
    • This technique is effective for studying nanoparticle dynamics and optical trapping.
    • It provides a reliable method for determining optical trapping threshold power.