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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Related Experiment Video

Updated: Jun 11, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Linear digital holography.

Yuma Sato, Yoshio Hayasaki

    Applied Optics
    |June 10, 2026
    PubMed
    Summary
    This summary is machine-generated.

    Linear digital holography (LDH) enables efficient complex-amplitude imaging of moving objects. This method offers low computational cost and high throughput for 2D shape measurement of laterally translating objects.

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    Last Updated: Jun 11, 2026

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
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    Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

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    Area of Science:

    • Optics and Photonics
    • Digital Imaging
    • Metrology

    Background:

    • Traditional digital holography (DH) can be computationally intensive.
    • Imaging laterally moving objects presents challenges in maintaining resolution and throughput.

    Purpose of the Study:

    • To introduce linear digital holography (LDH) for complex-amplitude imaging.
    • To demonstrate LDH's capability for measuring the 2D shape of laterally moving objects.
    • To highlight LDH's advantages in computational efficiency and measurement speed.

    Main Methods:

    • Utilizing a linear imager for data acquisition.
    • Applying one-dimensional (1D) Fourier transform for image reconstruction.
    • Employing 1D diffraction calculations to retrieve complex amplitude information.

    Main Results:

    • Successful complex-amplitude image retrieval of a moving object.
    • Demonstration of 2D shape measurement by scanning the object perpendicular to the sensor.
    • Achieved low computational cost and high measurement throughput compared to conventional DH.

    Conclusions:

    • LDH is an effective technique for imaging laterally moving objects.
    • The method provides a computationally efficient and high-throughput solution for 2D shape metrology.
    • LDH offers a promising alternative for real-time or high-speed imaging applications.