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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Related Experiment Video

Updated: May 26, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Partially coherent lensfree tomographic microscopy [Invited].

Serhan O Isikman1, Waheb Bishara, Aydogan Ozcan

  • 1Electrical Engineering Department, University of California, Los Angeles, California 90095, USA.

Applied Optics
|December 24, 2011
PubMed
Summary

Lensfree optical tomography provides high-throughput, micrometer-scale 3D imaging of biological specimens. This compact microscopy technique is ideal for lab-on-a-chip and resource-limited settings.

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Area of Science:

  • Biomedical Imaging
  • Optical Microscopy
  • 3D Reconstruction

Background:

  • Optical sectioning is crucial for detailed volumetric analysis of biological specimens.
  • Existing 3D microscopy techniques have limitations in throughput, size, or complexity.

Purpose of the Study:

  • To introduce and review lensfree optical tomography as a complementary 3D imaging modality.
  • To highlight the advantages of lensfree optical tomography for specific applications.

Main Methods:

  • Recording in-line holograms of specimens under partially coherent illumination at various angles.
  • Utilizing digital sensor-arrays to capture holographic data.
  • Computing pixel super-resolved tomographic images from recorded holograms.

Main Results:

  • Achieved micrometer-scale 3D resolution.
  • Demonstrated imaging of large volumes (10-15 mm³).
  • Developed a compact, lightweight, and high-throughput imaging platform.

Conclusions:

  • Lensfree optical tomography offers a novel approach to 3D biological specimen imaging.
  • The technique is well-suited for lab-on-a-chip devices.
  • It presents a valuable microscopy solution for resource-limited environments.