Measurement of microchannel flow with digital holographic microscopy by integrated nearest neighbor and cross-correlation particle pairing
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Updated: May 26, 2026

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
Published on: November 1, 2017
Yingchun Wu1, Xuecheng Wu, Zhihua Wang
1State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Abstract:
A micro digital in-line holographic particle tracking velocimetry (micro-DHPTV) system has been developed and applied to investigate the three-dimensional flow field in straight and Y-junction microchannels. The micro-DHPTV system comprises a cooled frame-transfer CCD camera and a double-pulsed laser. The processing algorithm introduced to evaluate the three-dimensional velocity is based on the combination of integrated cross-correlation and nearest neighbor matching algorithms, taking advantage of information from both the reconstructed particle field and the original holograms fringes patterns. Tests on simulated pairs of holograms show that the particles can be detected, located, and paired with high probability and accuracy. Results obtained in the straight and Y-junction microchannels show that the superimposed vector field is physically reasonable.

