Related Experiment Video
Updated: May 26, 2026

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
Published on: May 12, 2018
Identification of a thermally activated process in the Cy3 photobleaching mechanism
A L Jouonang1, P Didier, Y Mély
1Laboratoire de Biophotonique et Pharmacologie, UMR 7213, Université de Strasbourg 74 route du Rhin, F-67401 Illkirch, France.
Abstract:
Single molecule experiments were used to investigate the photobleaching mechanism of single Cy3 molecules encapsulated in large unilamellar vesicles. Analysis of thousands of individual fluorescence traces evidenced that the survival probability distribution is monoexponential with an average survival time depending on the laser intensity, the concentration of molecular oxygen, the temperature and the rigidity of the medium. Taken together, our data suggest that the photobleaching of Cy3 molecules is governed by two parallel pathways, namely photooxidation and a thermally activated structural rearrangement of the Cy3 molecules in their excited state.
More Related Videos
Related Concept Videos
Deactivation Processes: Jablonski Diagram
Cycloaddition Reactions: MO Requirements for Photochemical Activation
Photoreceptors and Visual Pathways
Thermal and Photochemical Electrocyclic Reactions: Overview
Photochemical Electrocyclic Reactions: Stereochemistry
Selection Rules: Photochemical Activation
The Photochemical Reaction Center

