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Related Experiment Video

Updated: May 26, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Quantification of methylated markers with a multiplex methylation-specific technology.

Hongzhi Zou1, Hatim Allawi, Xiaoming Cao

  • 1Exact Sciences Corporation, Madison, WI, 53719, USA. hzou@exactsciences.com

Clinical Chemistry
|December 24, 2011
PubMed
Summary

Quantitative allele-specific real-time target and signal amplification (QuARTS) efficiently quantifies methylated gene markers for colorectal cancer screening. This multiplex assay shows high accuracy in detecting colorectal neoplasia from healthy tissues.

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Last Updated: May 26, 2026

DNA Methylation: Bisulphite Modification and Analysis
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Published on: October 21, 2011

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics
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Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Area of Science:

  • Molecular diagnostics
  • Cancer biomarkers
  • Epigenetics

Background:

  • Aberrantly methylated genes are crucial for cancer diagnosis.
  • Multiplex detection of these markers is vital for clinical applications like colorectal cancer screening.

Purpose of the Study:

  • To describe a multiplex detection approach for quantifying methylated gene markers.
  • To evaluate the QuARTS technology for clinical applications in colorectal cancer screening.

Main Methods:

  • Quantitative allele-specific real-time target and signal amplification (QuARTS) was employed.
  • Multiplex QuARTS assays were developed to quantify methylated BMP3, NDRG4, VIM, and TFPI2 genes in 91 colorectal tissue DNA samples.
  • Percent methylation was calculated using ACTB as a reference gene.

Main Results:

  • QuARTS demonstrated linear detection down to 10 copies with no cross-reactivity.
  • The assay detected methylated genes at a 0.01% ratio.
  • At 95% specificity, the markers detected 84-92% of colorectal cancers and 68-88% of adenomas.

Conclusions:

  • QuARTS technology offers a promising method for quantifying methylated markers.
  • The selected markers effectively distinguish colorectal neoplasia from healthy tissues.