CD proteome and beyond - technologies for targeting the immune cell surfaceome.

Area of Science:

• Immunology
• Proteomics
• Systems Biology

Background:

• Cell surface proteins mediate crucial intercellular communication and regulate cell interactions within microenvironments.
• Current detection technologies offer limited views of the cell surface proteome, hindering comprehensive analysis.
• A significant challenge is the lack of high-quality antibodies for cell surface targets, impeding systems-level understanding.

Purpose of the Study:

• To highlight recent advancements in the identification and quantification of the cell surface proteome (surfaceome).
• To emphasize the potential of new mass spectrometry-based technologies for surfaceome analysis.
• To underscore the importance of the surfaceome as the cell's information gateway.

Main Methods:

• Review of recent developments in mass spectrometry-based technologies for proteomic analysis.
• Focus on qualitative and quantitative approaches for surfaceome profiling.
• Discussion of antibody limitations in current cell surface protein detection.

Main Results:

• Mass spectrometry now enables complementary approaches for comprehensive surfaceome analysis.
• Progress has been made towards the identification and quantification of the cell surface proteome.
• These advancements facilitate a systems biology view of the surfaceome.

Conclusions:

• The cell surface proteome is a critical subproteome for cellular information processing.
• Advancements in mass spectrometry are overcoming previous limitations in surfaceome research.
• Future research can leverage these technologies for a deeper understanding of cell surface functions in immunology and beyond.