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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

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Published on: March 20, 2015

Surface-enhanced Raman spectroscopy (SERS): progress and trends.

Dana Cialla¹, Anne März, René Böhme

¹Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Jena, Germany.

Analytical and Bioanalytical Chemistry

|December 30, 2011

Summary

Surface-enhanced Raman spectroscopy (SERS) offers high sensitivity for trace molecule detection. This review focuses on improving SERS reproducibility and exploring its advanced applications in chemical analytics.

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Area of Science:

Analytical Chemistry
Spectroscopy
Nanotechnology

Background:

Surface-enhanced Raman spectroscopy (SERS) provides molecular fingerprint specificity and single-molecule sensitivity.
SERS is a powerful technique for trace molecule detection in chemical and biochemical analysis.
Recent years have seen a surge in SERS research, indicating its growing importance.

Purpose of the Study:

To review recent progress and trends in SERS research over the last three years.
To address the challenge of low SERS signal reproducibility, hindering its routine use.
To highlight advancements in generating reproducible SERS-active surfaces.

Main Methods:

Review of literature focusing on SERS-active surface generation.
Discussion of single-molecule spectroscopy and enhancement factor calculations.
Summary of theoretical descriptions for SERS spectra.
Analysis of parameters affecting SERS spectra recording (laser power, integration time, concentration).
Exploration of SERS integration with complementary techniques like scanning probe microscopy and microfluidics.

Main Results:

Significant progress has been made in developing reproducible SERS-active surfaces.
Understanding of the theoretical underpinnings of SERS spectra is advancing.
The limits of sensitivity, including single-molecule detection, are being refined.
Synergistic combinations of SERS with other techniques expand its analytical capabilities.

Conclusions:

Improving SERS reproducibility is key to establishing it as a routine analytical technique.
Ongoing research in SERS theory and methodology continues to enhance its performance.
The integration of SERS with other advanced techniques broadens its application scope in various fields.