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Updated: May 26, 2026
A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening
Published on: October 16, 2015
Highly sensitive fluorescent detection of trypsin based on BSA-stabilized gold nanoclusters
Lianzhe Hu1, Shuang Han, Saima Parveen
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Abstract:
In this study, fluorescent metal nanoclusters are presented as novel probes for sensitive detection of protease for the first time. The sensing mechanism is based on trypsin digestion of the protein template of BSA-stabilized Au nanoclusters. The decrease in fluorescence intensity of BSA-Au nanoclusters caused by trypsin allows the sensitive detection of trypsin in the range of 0.01-100 μg/mL. The detection limit for trypsin is 2 ng/mL (86 pM) at a signal-to-noise ratio of 3. The present nanosensor for trypsin detection possesses red emission, excellent biocompatibility, high selectivity, and good stability. In addition, we demonstrated the application of the present approach in real urine samples, which suggested its potential for diagnostic purposes.
